lr ii recombinase (Thermo Fisher)
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Thermo Fisher
lr ii recombinase
Lr Ii Recombinase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lr+recombinase/pmc11735390-310-40-44
Average 90 stars, based on 1 article reviews
Lr Ii Recombinase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lr+recombinase/pmc11735390-310-40-44
Average 90 stars, based on 1 article reviews
lr ii recombinase - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: Unannotated Open Reading Frame in Saccharomyces cerevisiae Encodes Protein Localizing to the Endoplasmic Reticulum Article Snippet: Standard error of the percentage was calculated using the following equation: root-square (percentage*(100-percentage)/n) Saccharomyces cerevisiae strain 2x Master mix Q5 Hot Start polymerase (New England BioLabs) was used to amplify the construct containing Z 3 EVpr-mNG-YGR016C-A:HYG, using plasmid pARC0400 as template. .. Plasmid pARC0400 was made by Article Title: Correction: Reprogramming anchorage dependency by adherent‑to‑suspension transition promotes metastatic dissemination. Article Snippet: DNA, shRNA, and CRISPR/Cas9 constructs and ASTTetR and Ikzf1−/− cell line generation Candidate human AST genes were tagged with V5 and FLAG and subcloned into the gateway entry vector pENTR4 vector (Addgene, 17,425 and 17,423). .. The resulting subcloned vectors were recombined with destination vector pLentiCMV vector (Addgene, 17,293) using Article Title: Heterologous expression of the Glycine soja Kunitz-type protease inhibitor GsKTI improves resistance to drought stress and Helicoverpa armigera in transgenic Arabidopsis lines. Article Snippet: Kunitz-like protease inhibitors (KTIs) have been identified to play critical roles in insect defense, but evidence for their involvement in drought stress is sparse.. The aim of this study was to identify and functionally characterize a Kunitz-like protease inhibitor, GsKTI, from the wild soybean (Glycine soja) variety ED059.. Expression patterns suggest that drought stress and insect herbivory may induce GsKTI transcript levels. Article Title: A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules Article Snippet: The amplified fragment was then cloned into the pENTR-D-TOPO vector (Invitrogen), and the resulting entry clone (ENT_LjFtsH4) was confirmed by sequencing to ensure no PCR errors. .. The insert from the entry clone was transferred to a Gateway-compatible binary vector, pUbi-GW-GFP , using an Article Title: In planta transposon sequencing for virulence gene identification in Xylella fastidiosa Article Snippet: .. Then each complementation construct was recombined with plasmid pAX1/GW to create X. fastidiosa genomic insertion constructs using Expressing:Article Title: Adherent-suspension plasticity promotes the dissemination and colonization of circulating tumor cells Article Snippet: AST genes were tagged with V5 and subcloned into the pENTR4 vector (Addgene, 17425 and 17423) using standard molecular cloning techniques. .. The resulting subcloned vectors were then recombined with the destination vector pLentiCMV (Addgene, 17293) using Article Title: Paralog-dependent isogenic cell assay cascade generates highly selective SLC16A3 inhibitors. Article Snippet: .. HiBiT tag and/or mutations were introduced using site directed mutagenesis (New England Biolabs; oligonucleotides listed in Table S2) and pDONR221 vectors. cDNAs were cloned into gateway-compatible vectors pRRL (for lentiviral expression, EF1a promotor driven expression, described previously54) or pJTI (for transient transfection, Thermo Fisher Scientific) using Article Title: Correction: Reprogramming anchorage dependency by adherent‑to‑suspension transition promotes metastatic dissemination. Article Snippet: DNA, shRNA, and CRISPR/Cas9 constructs and ASTTetR and Ikzf1−/− cell line generation Candidate human AST genes were tagged with V5 and FLAG and subcloned into the gateway entry vector pENTR4 vector (Addgene, 17,425 and 17,423). .. The resulting subcloned vectors were recombined with destination vector pLentiCMV vector (Addgene, 17,293) using Mutagenesis:Article Title: Paralog-dependent isogenic cell assay cascade generates highly selective SLC16A3 inhibitors. Article Snippet: .. HiBiT tag and/or mutations were introduced using site directed mutagenesis (New England Biolabs; oligonucleotides listed in Table S2) and pDONR221 vectors. cDNAs were cloned into gateway-compatible vectors pRRL (for lentiviral expression, EF1a promotor driven expression, described previously54) or pJTI (for transient transfection, Thermo Fisher Scientific) using Clone Assay:Article Title: Paralog-dependent isogenic cell assay cascade generates highly selective SLC16A3 inhibitors. Article Snippet: .. HiBiT tag and/or mutations were introduced using site directed mutagenesis (New England Biolabs; oligonucleotides listed in Table S2) and pDONR221 vectors. cDNAs were cloned into gateway-compatible vectors pRRL (for lentiviral expression, EF1a promotor driven expression, described previously54) or pJTI (for transient transfection, Thermo Fisher Scientific) using Article Title: NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis. Article Snippet: The following chemicals were used for this study: 10 μg/μl cycloheximide (AC357420010; Thermo Fisher Scientific), 10 μm MG132 (NC9937881; Thermo Fisher Scientific), 1 μM TAK243 (30108; Cayman Chemical), 50 μM chloroquine diphosphate (C2301100G; Thermo Fisher Scientific), 3 μM MRT67307 (inhmrt; Invivogen), 5 mg/ml LPS (eBioscience), 1 mg/ml poly I:C (Thermo Fisher Scientific), 5U FastAP Thermosensitive Alkaline Phosphatase (EF0651; Thermo Fisher Scientific), 1 μM dTAGV-1 (Toris), and 10 μM BI605906 (50-203-0195; Thermo Fisher Scientific). .. To generate NAP1 rescue lines, NAP1 cDNA was cloned into pDONR223 and transferred into the pHAGE-N’-FLAG-HA-IRESpuro, pHAGE-N’-EGFP-Gaw-IRES-Blast, or pHAGE-C’-EGFPGaw-IRES-Blast vectors using Transfection:Article Title: Paralog-dependent isogenic cell assay cascade generates highly selective SLC16A3 inhibitors. Article Snippet: .. HiBiT tag and/or mutations were introduced using site directed mutagenesis (New England Biolabs; oligonucleotides listed in Table S2) and pDONR221 vectors. cDNAs were cloned into gateway-compatible vectors pRRL (for lentiviral expression, EF1a promotor driven expression, described previously54) or pJTI (for transient transfection, Thermo Fisher Scientific) using Construct:Article Title: Heterologous expression of the Glycine soja Kunitz-type protease inhibitor GsKTI improves resistance to drought stress and Helicoverpa armigera in transgenic Arabidopsis lines. Article Snippet: Kunitz-like protease inhibitors (KTIs) have been identified to play critical roles in insect defense, but evidence for their involvement in drought stress is sparse.. The aim of this study was to identify and functionally characterize a Kunitz-like protease inhibitor, GsKTI, from the wild soybean (Glycine soja) variety ED059.. Expression patterns suggest that drought stress and insect herbivory may induce GsKTI transcript levels. Article Title: In planta transposon sequencing for virulence gene identification in Xylella fastidiosa Article Snippet: .. Then each complementation construct was recombined with plasmid pAX1/GW to create X. fastidiosa genomic insertion constructs using |